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A ligation response is most productive in the event the sticky finishes are now stably annealed, and disruption of the annealing finishes would hence end in lower ligation efficiency. The shorter the overhang, the reduce the Tm.
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DNA ligaz I : bir olgunlaşmamış DNA ligasyonu gecikmeli telin sonra ribonükleaz H RNA primer kaldırdığı Okazaki parçaları .
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Probably the most successful ligation temperature for blunt-ended DNA would as a result be the temperature at which the greatest range of alignments can take place. The majority of blunt-finished ligations are performed at fourteen-twenty five °C overnight. The absence of stably annealed ends also ensures that the ligation efficiency is reduced, demanding a higher ligase concentration to be used.[ten]
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DNA ligase II: seems for use in repair service. It can be formed by substitute splicing of the proteolytic fragment of DNA ligase III and does not have its individual gene, therefore it is frequently regarded as virtually identical to DNA ligase III.